Truffle Application and Research Center is produced on order of truffle-infused seedlings.
Oak seeds from the region to be planted in truffle-infused seedlings are needed. Oak seeds from the relevant region are collected on time through the tree. The seeds are collected from the tree and should be careful not to crack the shells against the risk of contaminations
Germinating of seeds
Pre-disinfected seeds are planted in sterile vermiculite and left to germinate in 25ºC environment for 4 to 6 weeks. After this time, germinate seedlings are ready for inoculation.
Inoculation of Seedlings
Each truffle is diagnosed by morphological and molecular methods to ensure that the truffle is vaccinated before vaccination. After vaccination, 400 cm viols are sewn into a sterilized mixture.
Cultivation of seedlings
Seedlings taken to viols or tubes are taken to a fully controlled laboratory environment in case of contamination. It is developed at a temperature of 24ºC under sterile laboratory conditions for at least 8 months.
The biggest risks that can be encountered are;
To make sure that each of the truffle samples to be used in vaccination is the desired type.
This is because unwanted fungal spores cause contamination during and after inoculation by air or through contact.
It is possible to understand the risk of contamination from the following example.
There are approximately 20,000 to 2 million fungal spores in one cubic meter of air. Especially when mushrooms form hats, a single fungus produces about 40 million spores per hour for two days. Since most of these fungi partner with plants like truffles, there is a great deal of competition between them.
Therefore, vaccinated seedlings should be grown in a fully controlled environment without air contact with the external environment.
The greatest risk of contamination due to carelessness when growing seedlings is a fungus called Sphaerospora brunnea. This fungus can sometimes form orange ascocarp structures at the bottom of the seedlings. Formed from ascocarpas
Spores emitted from the formed ascomycetes infect the roots of all the seedlings in the greenhouse, forming their own ectomycorrhiza instead of inoculated truffles. In most cases, the ascocarp structure is very small, so the danger may not be noticed because it is not noticed. The mycoriza of Sphaerospora brunnea is morphologically similar to the mycoriza of Tuber melanosporum. The mycoriza of these two mushrooms can only be understood by highly experienced experts. Molecular study is needed for definitive diagnosis. If the seedlings in the greenhouse are infected with the spores of Sphaerospora brunnea, it will form mycoriza at the root and destroy Tuber melanosporum mycoriza. If these contaminated seedlings are planted in the field, it will result in a waste of time and money.